Electroporation or chemical
Kanamycin, Ampicilin, Chloramphenicol, Tetracycline, Spectinomycin
pUC, pMB1, p15a, RSF1010, RK2
Application specific, chemically defined and rich
Use standard alkaline lysis methods
Use standard USP & DSP equipment
>10K bp, ITR, toxic constructs
BSL-1 organism with significantly lower endotoxins
The NBx Platform™ strain is considered a safe bacterium, not associated with disease in healthy adult humans and like E. coli, requires Biosafety Level 1 containment.
Yes, inducing cell competence can be achieved by electroporation or chemical methods.
All most common plasmid replication origins can be utilized, including those from pUC, pMB1, p15a, RSF1010, RK2.
All most common plasmid selection markers can be used, including Kanamycin, Ampicillin, Chloramphenicol, Tetracycline, or Spectinomycin.
Yes, the NBx Platform™ uses proprietary, application specific media.
Yes, standard alkaline lysis methods can be used.
No, the strain can be handled and cultivated in standard USP and DSP equipment.