We offer a variety of production grades to suit your needs.
Each project is tailored to help our clients fulfill their research objectives.
Contact our team to learn more.
Research
High-quality
GMP
(Coming Soon)
Services
End-to-end provider of plasmid DNA production services –
projects tailored to client need.
Custom Plasmid DNA Construction
Construct new plasmid designs or modify existing plasmids using cutting-edge recombinant DNA techniques.
Plasmid DNA Manufacturing
Plasmid production to 50 milligram scale for research and pre-clinical applications using scalable, GMP-compatible, tech-transferable processes.
Rapid Plasmid Prep Services
Rapid plasmid preps at mini, midi, maxi, mega, and giga scale with turnaround times as short as 3 days and prices as low as $100 per sample.
Plasmid QC and Analytics Services
Comprehensive array of analytical testing services for plasmid DNA quality control.
DNA Sequencing Services
We provide NGS services for amplicons, plasmids, and microbial genomes with prices as low as $60 per sample.
Plasmid Construction
Denovo synthesis of complex plasmid designs, including large plasmids, poly-A tails, and ITRs.
Cell Line Generation
Transformation of plasmid production strain and preparation of Research Cell Bank.
Fermentation
Scale up cultivation of producing cell line in animal-free process in flasks or bioreactors.
Downstream Processing
Cell lysis, clarification, chromatographic purification processes, formulation, and vialing.
Analytical Characterization
Complete QC analysis of manufactured material (see available methods below).
Delivery
Plasmid material shipped to client on dry ice along with Certificate of Analysis.
Plasmid Construction
Denovo synthesis of complex plasmid designs, including large plasmids, poly-A tails, and ITRs.
Cell Line Generation
Transformation of plasmid production strain and preparation of Research Cell Bank.
Fermentation
Scale up cultivation of producing cell line in animal-free process in flasks or bioreactors.
Downstream Processing
Cell lysis, clarification, chromatographic purification processes, formulation, and vialing.
Analytical Characterization
Complete QC analysis of manufactured material (see available methods below).
Delivery
Plasmid material shipped to client on dry ice along with Certificate of Analysis.
| Research | High-quality | GMP | |
|---|---|---|---|
| PROJECT MANAGEMENT | |||
| White-glove service with best-in-class project management capabilities | x | x | x |
| PLASMID CONSTRUCTION | |||
| Scale up of existing plasmid constructs | x | x | x |
| DNA synthesis & cloning services for new plasmid construction | x | x | x |
| UPSTREAM PROCESS | |||
| Production of research cell bank | x | x | x |
| Production of master cell bank | x | ||
| Animal-free media formulations | x | x | x |
| Shake flask production (multiple scales) | x | x | x |
| Production in bioreactors (multiple scales) | x | x | |
| DOWNSTREAM PROCESS | |||
| "Established downstream processes producing quality, transfection-grade material" | x | x | x |
| Endotoxin removal | x | x | x |
| "Scalable, industry standard downstream process" | x | x | |
| Animal-free downstream process | x | x | |
| ANALYTICS | |||
| Analytical testing services | x | x | x |
| Certificate of Analysis | x | x | x |
| QC Product release testing | x | x | |
| DOCUMENTATION AND QUALITY ASSURANCE | |||
| Master Batch Record | x | x | |
| Full traceability | x | ||
| Production in GMP-certified suite | x | ||
| Physical project segregation in dedicated suite | x | ||
| Production under QA oversight | x | ||
We ensure protection of your data and IP
- All data is stored in a secured, cloud-based, GxP-rated electronic system.
- All work is performed in North America.
- All projects are performed under NDA.
Your plasmid will arrive:
- Manufactured in accordance with established Standard Operating Procedures.
- Shipped on dry ice (use of traceable temperature monitoring devices on request).
- Meeting the quality specifications established at the start of the project.
- In the agreed-upon quantity.
- Formulated in your desired buffer and prepared in aliquot sizes of your choice.
- With a Certificate of Analysis (CoA) listing the results from the QC analysis.
We take pride in the quality of our products.
Novel Biotechnology offers a full suite of analytical tests to evaluate various aspects of plasmid quality.
Analytical testing packages can be tailored to each project need.
| QC Analysis | Methodology |
|---|---|
| Concentration | UV absorption |
| Purity | A260/A280 Ratio |
| Appearance | Visual inspection |
| Plasmid topology | Agarose gel densitometry |
| Restriction Digest | Digestion + agarose gel |
| Plasmid sequence | NGS |
| Residual host DNA | Agarose gel |
| Residual RNA | Agarose gel |
| Residual protein | BCA assay |
| Endotoxin contamination | LAL assay |